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Freezing creates ice crystals that physically damage the peptide molecular structure, destroying its ability to bind to GLP-1 receptors

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The following primary antibodies were used in this study: Sigmar1 (1:100, 61994, Cell Signaling) and pCREBSer133 (1:100, 9198, Cell Signaling)

However, tumor cells can develop radioresistance through multiple mechanisms including DNA damage repair, antioxidation, cell cycle arrest, accumulation of cancer stem cells (CSCs), and immunosuppressive microenvironment formation [2-4], leading to cancer recurrence and metastasis after radiotherapy

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