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Description
Following autophagosome formation, the lysosomal membrane and the autophagosomes outer membrane fuse to produce an autophagic lysosome, which enables a variety of enzymes to break down the contents, including proteins, nucleic acids and lipids (142)

This cycle primarily involves -glutamyl peptidase (GGT), the only enzyme that catabolizes GSH, GSSG, and GS-X, as well as dipeptidyl peptidase (DP)

reported that the substitution of sulfur with Se in enzymes bearing cysteine amino acid moiety leads to a substantially enhanced catalytic activity [38]

Buvelot, H., Jaquet, V

[DOI] [PMC free article] [PubMed] [Google Scholar] 93.Kumar A., Sehgal N., Kumar P., Padi S.S.V., Naidu P.S
For labelling ES2, a cysteine was added to the N terminus and BODIPY FL maleimide (ThermoFisher, B10250) was used to conjugate the fluorophore to the peptide following the manufacturer's protocol