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In vitro BP incorporation was performed at 37 C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% -mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 C for 10 min

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The combination therapy enhances intracellular Fe 2+ accumulation and downregulates GPX4 expression, thereby promoting ferroptosis

This therapeutic effect is achieved through the cleavage of disulfide bonds [33] within mucoproteins (strongly cross-linked mucins), [34] thereby decreasing the mucus viscosity and facilitating its clearance from the respiratory tract

During mastication they are enzimatically converted into thiocyanates, goitrin metabolites, that cause a competitive inhibition of the sodium/iodide symporter, thus inhibiting the thyroid hormones synthesis and possibly causing hypothyroidism [156]