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This is one reason head-to-head binding assays dont always match head-to-head secretion experiments and why researchers occasionally get surprised when GHRP-6 outperforms GHRP-2 in certain model contexts despite lower receptor binding affinity on paper

doi: 10.1093/carcin/7.9.1463

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More intriguingly, chelation of endogenous of copper with bathocuproine decreased spontaneous spiking activity, suggesting that endogenous copper regulates spiking activity in active hippocampal networks (Maureira et al., 2015)
