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Centrifuge the supernatant again for 30 min at 18,000 rpm at 4 C to get a clear soluble lysate
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doi: 10.1039/D3FO02824K 10 OguzSHYildizBO
The supernatant was collected and filtered through a 0.22-m filter
and Buettner, G.R., Free Radical Biol
