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Use 5-10 bed volume of Elution buffer (added GSH) to elute, collect in fractions, collect one tube for each bed volume, and test separately, which can not only ensure that all binding target proteins are eluted, but also obtain high-purity and high-concentration proteins

Cell 149 , 16351646 (2012)
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Glutathione Glutathione is an antioxidant produced primarily by the liver

The chemical composition of plant oils is more complex with many more possible allergens or irritating components, while mineral oil is simple, pure and sensitivity to it is extremely rare