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Gs recruitment assay G protein recruitment to CTR or individual AMYR subtypes was measured in COS-7 cells (ATCC, CRL-1651) transfected with c-myc-hCTRaleu-Rluc8:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP1 Nluc:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP2-Nluc:Gs-Venus:G1:G2 or c-myc-hCTRaleu:RAMP3-Nluc:Gs-Venus:G1:G2 at a ratio of 2:0.1:1:1:1, in a total of 51 ng DNA per well using PEI at a ratio of 1:6 of DNA to PEI

Tap out air bubbles and push them back into the vial

Pediatr Res (2005) 58(5):94652

IV therapy is the least convenient, involving hours at a clinic and often a higher cost per session

Additionally, scientists examine its influence on blood pressure and heart rate regulation pathways

Normal dermal fibroblasts were seeded into a 96-well cell culture plate and have been grown to confluence in high glucose media, supplemented with 10% fetal calf serum for around 3 days