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Then the pellets were stained for 20 min in 100 l assay buffer containing 5 g ml 1 propidium iodide (Sigma-Aldrich, P4170) and 3 l Annexin VFITC (BioLegend, 0640906)

Some chronic headaches and migraine attacks have serious underlying conditions and stem from nerve problems in your head, neck, or face or pressure that inexplicably builds up in your head