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After isolation, the nuclei were analyzed using the Nexcelom Cellometer Auto2000 with the AOPI fluorescent staining method, followed by tagging of open chromatin regions with Tn5 transposase
Cells were then washed in PBS and analyzed by flow cytometry using a BD Accuri C6 + instrument (BD Biosciences, San Jose, California), with 10,000 live cells gated for each replicate
