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For unmodified SpyCatcher mutants, all variants with cysteine replacing hydrophobic or aromatic amino acids had decreased melting temperatures, as one would expect from modifying amino acids directed towards the hydrophobic core (Fig
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2 and 3), which seems to be inconsistent with the previously defined role of Glu as a critical wound signal that activates plant defense response 11
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J Neurosci 24: 886894, 2004
