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The release of ALT into hepatic and vascular culture supernatants was analyzed using the Human ALT ELISA Kit (Abcam)

J Per Nerv Syst 2008

Minnesota Surgical Society, Fall Conference, 2009

The wash buffer was based on DMEM (ThermoFisher) supplemented with penicillin/streptomycin (ThermoFisher) and 2% fetal bovine serum (FBS) (Sigma-Aldrich) as described by Broutier et al

The patient was discharged from the hospital 4 h after the last injection

Acknowledgements Figures 1, 2, 3 and 4 in this review were created using BioRender.com ( Funding This work was supported by the National Natural Science Foundation of China [grant numbers 81973498 and 81774283], National Science Foundation For Distinguished Young Scholars [grant numbers 82125037], the Joint Open Project of Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica and Yangtze River Pharmaceutical Group (No