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Nicotinamide N-methyl transferase (NNMT): an emerging therapeutic target

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Cells were manually scraped, spun at a 100 g for 5 min, the pellets washed with PBS and then homogenized in a Tris salt buffer, transferred to ultracentrifuge tubes and spun at 163, 200 g and 4 C for 30 min to extract the soluble fraction

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Scientific Reports 10:16749

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