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Cell culture and seeding for experiments NIH-3T3, U937 and neuroblastoma cells, SH-SY5Y, were obtained from ATCC and were maintained in HyQ-DMEM media with 5% Fetal Clone III, (Gibco/BRL), 50 U/ml penicillin, and 100 g/ml streptomycin in 100 mm Petri dishes
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and complementary ingredients like sulforaphane that boost glutathione recycling Dosage Effective doses typically range from 250-500mg daily

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