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Approximately 6% of people under age 60 and 20% of people over age 60 are vitamin B12 deficient

Successful hydrophobication of GSH-AuNCs and their excellent colloidal stability in organic solvents encouraged us to evaluate their encapsulation into PLGA nanoparticles using a modified nanoprecipitation method and the emulsion-evaporation method, as detailed earlier in the manuscript

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After those membranes were incubated with 5% fat-free dry milk-TBS buffer for 1 h at room temperature and then washed in TBST buffer (phosphate-buffered saline containing 0.1% Tween 20) for 5 min and then in TBS buffer twice for 10 min

doi: 10.1016/s0196-9781(99)00208-9