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The slices were rinsed and placed into a chamber embedded in the stage of an Olympus BX51WI microscope (Olympus) coupled to a 12-bit CCD fluorescent camera (SensiCam, PCO imaging)

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It is important to note that whilst B12 deficiency can cause nerve pain, not all nerve pain is caused by B12 deficiency

Protein-rich nutrition with many vitamins and minerals promotes the formation of glutathione in the body