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For in vitro cell culture analysis the composition was prepared in sterile DMEM HG medium and did not contain preservatives

The relationship may be causal

Furthermore, cyclodextrins ensure a better distribution

[16] [17] Whereas the latter directly act on the dopamine transporter (DAT) to inhibit the reuptake and/or induce the release of dopamine, bromantane instead acts via indirect genomic mechanisms to produce a rapid, pronounced, and long-lasting upregulation in a variety of brain regions of the expression of tyrosine hydroxylase (TH) and aromatic L-amino acid decarboxylase (AAAD) (also known as DOPA decarboxylase), key enzymes in the dopamine biosynthesis pathway

All procedures should comply with local regulations, safety standards, and organizational requirements

Fried MW, et al