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Description
Spermatogenesis was categorized according to Johnsens score ( 2.8 Immunohistochemical approach Five m-thick sections were obtained using paraffin blocks

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Briefly, the Parafilm M was cut according to the size of the patches and stacked to obtain an eight-layer film with ~ 1 mm thickness (~ 110 m per layer)

As seen in Figure 7B, the variable importance in projection (VIP) scores revealed that sperm qualities (sperm count, morphology, and motility), oxidative stress (MDA), and inflammatory markers (IL-6, TNF-, IL-1, and NF-) were the most influencing variables in the present study

This work was supported by the Natural Science Foundation of Hubei Province (2024AFB391)
doi: 10.1089/ars.2014.5994