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To confirm GF status, microbiological assays were performed on a monthly basis by culturing feces, bedding, and drinking water in thioglycollate medium (DIFCO, Camarillo, CA)

PMID: 197 MiyasoHOgawaYItohM

This was demonstrated by elevated transcript levels of autophagy-related receptors NIX and Ras Homolog Enriched in Brain (RHEB), as well as autophagy genes such as Atg9 and Atg12 , in ovarian granulosa cells of females with PCOS or in mice with PCOS induced by DHT (137, 138)

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