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Garg, Disorders of vitamins and cofactors, in Biomarkers in Inborn Errors of Metabolism, Elsevier, 2017, pp

For inhibition of transcription, HepG2 cells were treated with actinomycin D (5 g ml 1 , Sigma-Aldrich, A1410) for 24 h and lysed for qRTPCR analysis as described above
Keratin is a type of protein thats present in surface cells
Another common mutation of the MTHFR gene is the transversion of adenine to cytosine at position 1298

So that's kind of a mindset

Differentially expressed genes in GSTP1 knockdown cells were identified by Illumina HT-12 expression arrays and qRTPCR analysis, and altered pathways predicted by MetaCore (GeneGo) analysis