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Bedekovic, V., et al

Jerne explained the rationale behind his method in his diary in March 1962 as follows: 'Antibody molecules diffusing from a single cell into agar containing red cells and complement should lyse [puncture] a sufficient number of surrounding red cells to produce a visible plaque'(Jerne, 1981)

It is important to understand, however, that environmental factors were often the most dominant in preindustrial societies

A comprehensive panel of antibodies was utilized for Western blotting, targeting phosphorylated and total forms of ERK1/2, p38 MAPK, JNK1/2, and NF-B p65 (Ser536), with -actin serving as a loading control