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The obtained tissue explants were cultured in DMEM/F-12 (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 2% penicillin/streptomycin (Sigma, Germany) and incubated at 37 C and 5% CO 2 until the outgrowing cell confluence reached 80%

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Gallbladder-related adverse events, including cholelithiasis and cholecystitis, have been reported more frequently in patients receiving semaglutide compared to placebo in clinical trials, with an incidence of approximately two to three percent

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