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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

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Santoro MM

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Thioredoxin-1 (Trx-1) is a multifunctional molecule that has anti-inflammatory properties in human tissues and plays significant neuroprotective roles in AD (Jia et al., 2024)

cremoris and was not related to the intracellular GSH content [20]