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doi: 10.5588/ijtld.12.0512

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Unless indicated, erythrocytes were cultured as previously described at 2% hematocrit in complete media (incomplete media supplemented with 27mM sodium bicarbonate, 11mM glucose, 5mM HEPES, 1mM sodium pyruvate, 0.37mM hypoxanthine, 0.01mM thymidine, 10g/mL gentamicin, and 0.5% Albumax I (Thermo-Fisher) under 5% O 2 /5% CO 2 /90% N 2 atmosphere at 37C ( Plasmodium falciparum Growth Inhibition Assays Asynchronous cultures diluted to 1% parasitemia in 100L wells were subjected to drug dilutions, solvent controls, and untreated uninfected erythrocyte controls in technical duplicates

Briefly, 0.1 g seed sample was ground in liquid nitrogen, homogenized with 3 mL of 100 mM phosphate buffer solution (PBS) buffer (pH 7.8) and centrifuged at 10,000 x g for 20 min at 4 C

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