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ELISA for FcRn binding analysis 96-well EIA/RIA Clear Flat Bottom Polystyrene Microplates (Corning) were coated overnight at 4 C with 8 g/mL (100 L/well) of a recombinant Fc-engineered human IgG1 mutant (YTE/KF) 98 , with specificity for 4-hydroxy-3-iodo-5-nitrophenylactic acid, diluted in DPBS (pH 7.4) (Sigma-Aldrich)

In our investigation we used nestin, -tubulin III, GAP43 and doublecortin, because these genes and proteins play a key role in neurogenesis

doi: 10.1111/jocd.14626

This is because the carboxylate groups of these acyl-CoA molecules can undergo endogenous nucleophilic attack on the thioester bond of coenzyme A, resulting in the formation of a cyclic anhydride that is more reactive than the parent acyl-CoA, thereby facilitating a more effective non-enzymatic modification of proteins

On the next day, the cells were exposed to BPC-157 (1 g/mL, 5 g/mL, and 10 g/mL)