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Where resources preclude universal screening, a risk-stratified approach, screening patients with a family history of aminoglycoside-related hearing loss or those facing prolonged or repeated treatment, may represent a pragmatic intermediate step (McDermott et al., 2022b)

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For example, 10% final concentration of DMSO in the PCR mixture with Phusion decreases primer annealing temperature (i.e., primer melting temperature) by 5.56.0 C (9.910.8 F)

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Anal Chem 87:706715

Decreased synaptic plasticity and function, increased apoptosis, and impaired neuronal signaling were reported