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Description
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: The Keio collection
Nagy et al., 2024)
Kenji Sato, Email: [email protected]
Step 1 To a 25 mL three-necked flask was added compound 17a (150 mg, 0.42 mmol), compound 17b (134 mg, 0.46 mmol) and acetonitrile (2 mL), and the reaction mixture was cooled to 10 C., then T 3 P (350 mg, 0.4 mol) was slowly added dropwise
I., Said, E
Important Administration Tips Begin on a small dose and observe reaction
