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This is not a theoretical concern it is a documented pharmacological phenomenon with significant implications for protocol design

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[DOI] [PubMed] [Google Scholar] Parenchymal Disorders 1.Rutherford A, Chung RT

45 In addition to this spleen-mediated mechanism in endotoxaemia, efferent vagus nerve endings might directly regulate the immune function by releasing acetylcholine, without the requirement for either signalling along the splenic nerve or T cells (Figure 1, Figure 2)

TLR2 is most likely to identify polysaccharides containing galactose and glucose, which regulate immunomodulatory functions [51,52]