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Adding paraphenylenediamine (PPD) to henna turns the henna black

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doi:10.1016/j.pnpbp.2006.06.012

Tian and Tang, 2022

Pellets were resuspended in lysis buffer (50 mM Tris pH 8.0, 500 mM NaCl, 5% (v/v) glycerol, 15 mM imidazole, 4 mM BME and 1 tablet of cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 11873580001)), with 1 ml of lysis buffer per 240 ml of overnight culture