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After sorting, neutrophils were centrifuged at 500 g at 4 C and resuspended in RPMI-1640 medium with 10% FBS for subsequent culture, stimulation and functional assays

LL-37 is a key player of the innate immune system and acts to perforate the cell wall and cytoplasm of bacteria leading to their death, a function achieved by its oligomerization into a hexameric channel shape with a charged core that is necessary for the physical attack of the bacterial cell (Sancho-Vaello et al., 2020

HamLin WE, Riebe KW, Scothorn WW, et al

Enterohepatic circulation and renal reabsorption of cobalamin with the participation of the receptor protein (megalin) contribute to the sparing management of this vitamin and cause the excretion of excess absorbed cobalamin to be significantly limited

Pouyssgur, J

7 , ra1 (2014)