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The following primers were used: AgRP (forward: ctttggcggaggtgctagat
Yes, in excessive amounts, it can lead to digestive issues and nutrient imbalances
In the heat stimulation, there were significant differences for the main effect in both weeks and groups (p = 0.0002 and 0.0185, respectively)
28-30 As its effects on food intake are transient, it has been said that the effect of leptin on energy expenditure could be the most prominent in terms of body weight regulation
Cells were washed with PBS and then blocked for 30 minutes with 1% Bovine Serum Albumin (BSA) in PBS
