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Beads were then washed four times with wash buffer (50 mM TrisHCl pH 7.4, 150 mM NaCl, 0.1% Triton X-100) and then twice with wash buffer lacking Triton X-100

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Wong CS, Chang YC, Yeh CC, Huang GS, Cherng CH (2002) Loss of intrathecal morphine analgesia in terminal cancer patients is associated with high levels of excitatory amino acids in the CSF

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