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After completion of the baseline MR scan, participants were randomly assigned in a ratio of 5:2 to either the intervention or control group using a computer-generated random-sequence allocation program

The peptide is frozen and then placed under a vacuum, which causes the frozen water to sublimate (turn directly from solid to gas)

The eluates were passed through a 10 kDA cut-off filter and concentrated by centrifugation at 14,000 g for 20 min at 4 C

With respect to zinc supplementation, the expression of ZnT1 in the intestinal villi has increased in animal studies [30,31]

Serum Malondealdehyde (MDA), uric acid, bilirubin, protein carbonyl (PC) and plasma vitamin E levels were determined as the markers of oxidative stress