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Description
Introduced in the 1980s, organotypic slice cultures were traditionally cultured using the roller-tube method but have since been optimized to culture via the air-liquid interface method, which allows slices to simultaneously receive nutrients from the culture medium and humidified air, facilitating long-term viability (Ghwiler, 1981
Later on, it was discovered that he was suffering from pernicious anemia , which is a severe form of B12 deficiency

Microorganisms 8(2):280

Many patients repeat cycles 2-4 times per year as part of a comprehensive longevity program

(2025) Artificial-intelligence-based smart toothbrushes for oral health and patient education: A review

However, challenges such as high initial costs and limited acceptance decrease the generalization of bariatric surgery as a strategy to lower the obesity impact on public health
