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Model building and refinement An initial model of Ycf1 was built using the SWISS-MODEL server 46 and PDB ID: 6JB1 as a template 47

Utilizing recombinant human FLAG-tagged PEX7 instead of [ 35 S]H 6 PEX7 in our native PAGE assays, we observed the same complexes, as well as the previously reported PEX5PEX7PHYH complex 33 (Extended Data Fig

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