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doi: 10.1159/000108298

[DOI] [PMC free article] [PubMed] [Google Scholar] 30.Wu, X., Li, X., Fu, Q., Cao, Q., Chen, X.et al

A functional genetic variation of adenosine deaminase affects the duration and intensity of deep sleep in humans

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RT-PCR reactions were performed in a total volume of 25 l containing 25100 ng of total RNA, 200 m of each dNTP, 1.5 m MgCl 2 , 25 pmol of each primer, 2.5 U of AMV RT and 2.5 U of TFL DNA polymerase (Access RT-PCR System, Promega)