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An early study using mitochondria-depleted cells demonstrated that although TNF-induced ROS production accompanied necroptosis, mitochondrial ROS was not required for RIPK3-dependent cell death execution
Subsequently, 10 L of CCK-8 reagent mixed with 90 L culture medium was added to each well and incubate for 1 h
Is AOD-9604 safe for long-term use
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5J) and enrichment of the ROS pathway in KO cells (Supplementary Fig
