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The homogenate was centrifuged for 15 min, at 12,000 g and 4 C and the supernatant was collected and stored at 80 C until use

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Savage MO, Blum WF, Ranke MB, Postel-Vinay MC, Cotterill AM, Hall K, Chatelain PG, Preece MA, Rosenfeld RG

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