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A similar approach known as protein misfolding cyclic amplification was used by Soto and colleagues 89

Involvement of cyclooxygenase-2 and prostaglandins in the molecular pathogenesis of inflammatory lung diseases

All samples were incubated with Alexa Fluor 488 phalloidin green fluorescence conjugate (1:200), as a marker of the cytoskeleton actin and with TO-PRO staining to stain the nuclei [42]

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Many doctors unfortunately are completely unaware of the lethal nature of B12 deficiency and pernicious anemia

89 Moreover, fetuin-A-deficient mice spontaneously developed significant myocardial calcification, characterized by myocardial stiffness, cardiac remodeling and fibrosis, and diastolic dysfunction