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2018 Jul 1;1(3):72

Protein samples were separated by 412% SDS-PAGE, transferred onto PVDF membranes (GE Healthcare, Milan, Italy), and incubated with antibodies targeting and Alpha-Smooth Muscle Actin (catalog n A2547, Sigma-Aldrich)

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In addition to the gene expression, the mucus thickness may be of interest since the mucus layer may not cover the entirety of the intestine as reported in rats (131)