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These differences ensure uneven electron distribution, further confirming glutamines polarity

doi: 10.1074/jbc.M110.116814
Depending on the goals and specific needs for iPSC-HLCs as an in vitro model system, a dedifferentiated state might be preferable, for example, during investigation of liver regeneration mechanisms

Sinzel, M

26 , 151525 e4 (2019)

Once the lysate had been loaded, the column was washed with 20 column volumes (CV) wash buffer, and the His-tagged TRFH domain was eluted using a linear gradient from 0 to 100% elution buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 1 mM TCEP, 300 mM imidazole) over 25 CV
