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Briefly, 25-30 million cells were collected and washed twice with cold saline (0.9% NaCl), scraped into 1 ml of cold KPBS, and pelleted via centrifugation at 1,000g for 1.5min at 4C

Food Chem Toxicol 126:16

J Biosci Bioeng 123(5):555561 Orumets K, Kevvai K, Nisamedtinov I, Tamm T, Paalme T (2012) YAP1 over-expression in Saccharomyces cerevisiae enhances glutathione accumulation at its biosynthesis and substrate availability levels

(2014) Phosphorylation of KasB regulates virulence and acid-fastness in Mycobacterium tuberculosis

282 2743627446 10.1074/jbc.M704976200 [DOI] [PubMed] [Google Scholar] Notkola I

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