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AAV-PHP.eB viruses (1 10 12 vg/mL, 100 L total volume, diluted in sterile saline) were slowly injected into the lateral tail vein using a sterile 2730-gauge needle attached to 0.5 mL syringe

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Plates were mixed, briefly centrifuged and left at room temperature for 8 h before counting in a MicroBeta plate counter (Perkin Elmer)

Romboutsia lituseburensis JCM1404 supplementation ameliorated endothelial function via gut microbiota modulation and lipid metabolisms alterations in obese rats