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Furthermore, elevated ROS levels can damage DNA, leading to oxidative DNA damage

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On day 40 after 72 h of induction, cells were washed three times with phenol-red free Williams medium E and a probe cocktail composed of midazolam 5 M (CYP3A4), S -mephenytoin 50 M (CYP2C19), bupropion 50 M (CYP2B6), flurbiprofen 10 M (CYP2C9), and phenacetin 10 M (CYP1A2) in phenol-red free Williams medium E was incubated for 3 h