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For gain-of-function studies, M0 macrophages were seeded in a Seahorse XF96 cell culture microplate (50,000 cells per well), transfected with either an empty vector or an HSL-encoding plasmid, and cultured for 24 h

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Recovering from injury or surgery can present formidable challenges, often accompanied by muscle shutdown and inflammation in the soft tissues

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The mechanical stress is catastrophic to their structure