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Consuming more than 3 units/day (12-13 g alcohol/unit) increases the likelihood of cirrhosis in both men (OR 4.3) and women (OR 5.7), with a multiplicative interaction between alcohol intake and HBsAg positivity further elevating the risk (8)

The efficiency of each primer was calculated using qPCR assays of cDNA dilution series and plotting the log10 of the concentration versus the crossing point threshold (C(t)) values and using the primer efficiency equation, (10(1/Slope) 1) 100) (Supplemental Table S11)

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