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The lysate with LLO was loaded onto 30 ml Ni-NTA Superflow resin (QUIAGEN, Germany) packed in a XK 16/200 column (GE Healthcare, USA), washed with wash buffer (20 mM MES/NaOH pH 6.5, 150 mM NaCl), and eluted with an imidazole gradient in 20 mM MES/NaOH pH 6.5, 150 mM NaCl, with 500 mM imidazole as the final concentration

doi: 10.1186/s13045-024-01541-w 108 LiX.ZhangS.GuoG.HanJ.YuJ

1a for absolute quantification values

Para algunas personas con restricciones dietticas especficas, la suplementacin puede ser una opcin para cubrir sus necesidades

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