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Briefly, 500 bp of the upstream and downstream region of each gene were amplified by PCR and the respective PCR products were annealed via overlap extension PCR

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3a, the nucleus pulposus with Pfirrmann grade 1 was uniform and well demarcated with the annulus, while the nucleus and annulus in the sample with Pfirrmann grade 34 had poorly defined margins

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Seed germination ecology of Portulaca oleracea L.: an important weed of rice and upland crops

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