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the published literature commonly recommends single-use aliquots over repeated freeze-thaw of a single stock

Key points Drug-tolerant persister (DTP) cells are phenotypically heterogeneous, with diverse characteristics such as slow-proliferating or quiescent phenotypes, and a range of metabolic adaptations, cell identity switches and immune-evasion mechanisms

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Genome-wide knockout, knockdown, and activation screens are now systematically revealing both canonical and unexpected regulators of autophagy, mitophagy, UPS function, and ER stress adaptation

Safe use in pregnant animals or animals intended for breeding has not been proven

Typically, it is offered via expensive IV drips and shots or through a sticky liposomal supplement