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Risk factors include (Martin Arias et al, 2017

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Endothelial cells were then released from beads and reselected with magnetic beads conjugated with anti-mouse Podoplanin anti-PDPN (8.1.1) (1: 50, BioLegend) in order to obtain CD102+ and Podoplanin+ cells

For example, the target cell may be very rare in the sample and comprise only 0.01% of the sample or the entire sample (i.e., 100%) may comprise the target cell of interest
