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An equal volume of ice-cold neutralization buffer (500 mM HEPES, pH 8.0) was added to the acid supernatants

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[DOI] [PMC free article] [PubMed] [Google Scholar] 202.Polverino F., Wu T.D., Rojas-Quintero J., Wang X., Mayo J., Tomchaney M., Tram J., Packard S., Zhang D., Cleveland K.H., et al

No visible sterilisation protocol is in place
